Diterpenoids as potential anti-malarial compounds from Andrographis paniculata

Abstract Background The objectives of the current study are to evaluate traditionally used medicinal plants Andrographis paniculata for in vitro anti-malarial activity against human malarial parasite Plasmodium falciparum and further characterize active extract A. using spectroscopic chromatographic methods. Results chloroform displayed with IC 50 values 6.36 μg/ml 3D7 strain 5.24 K1 strains respectively no evidence significant cytotoxicity mammalian cell line (CC > 100 μg/ml). LC-MS analysis led identification 59 compounds based on their mass spectrometric features (a total 35 present positive ion 24 negative mode). We have identified 5 flavonoids 30 as diterpenoids mode, while mode all were diterpenoids. Characterization most promising class compound HPLC-LC-ESI-MS/MS was also undertaken. Conclusions results undoubtedly validate traditional use treatment malaria. from IGNTU_06 potential that need be purified analyzed drug development programs. Graphical abstract